Can anyone help me with cerebrospinal fluid cytocentrifugation? What are the best speed/time? Do I need to add 22%BSA or autologous serum?

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Gaber Ramadan

Hi Guilherme

Concentrate the cells using regular centrifuge (500 - 650 rpm) for 3- 5 minutes. Then, resuspend in 0.5 ml saline. Finally, use Cyto•Fluid Fix™ and cytospin slides as the cerebrospinal fluid has sparse or no cells. Centrifugation at 650 rpm for 2 minutes. Stain after draying.

Arjan Tibbe

Did you ever consider filtraton instead of cytospinning? Filtration simplifies the procedure considerably with and has a very high yield. Take a look at www.vycap.com to find out if filtration is working for your purpose.