I'm studying stability of certain enzymes with their mesophilic and thermophilic homologues using MD Simulation methods. Using the information obtained from the study I'm trying to design point mutations which would help in increasing stability. Is it possible to suggest some appropriate methods of studying such mutants quantitatively. e.g. in terms of melting temperatures or something like that?
Dear Saugata,
you can use Pmut scan in Rosetta for this purpose. Please go to the google and download it on your machine and have fun with it. I hope that you will be able to address your specific question.
All the best
-Yogendra
A similar discussion is in the link below
https://www.researchgate.net/post/Anyone_know_if_its_possible_to_use_simulated_annealing_to_estimate_the_Tm_of_a_3D_protein_structure
You may also take a look at this paper for some ideas on how structural factors affect thermal stability
http://www.sciencedirect.com/science/article/pii/S0076687901344865
You can try Pretherm to predict thermal stability
http://www.biomedcentral.com/1471-2105/11/370
- Badges
- Science topic