Thanks, Ramyar. The database is available, and I have tried with three different designer-software. Problem is that these primers have either very high CG content, or very high self-complementarity. So it is really hard to design the "perfect" primer, which shows nice melting curve without byproducts.

Thank you, Dr. Fiedler. I'll try them. Pity, that the company does not reveal the sequences

Hi, Anna,

I think you can trust the commercialized primers. And, if you wanna get the primers' sequences, you can insert the PCR product into a T-A vector and just sequence it. It sounds reasonable, right?

Everyone, many thanks for your help!