The enzymes are:

1. 4-hydroxy-2-oxovalerate aldolase

2. Carbazole 1,9a-dioxygenase

3. 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase

4. 2-hydroxy-6-oxo-6-(2'-aminophenyl)-hexa-2E,4Z-dienoate hydrolase

5. Anthranilate 1,2-dioxygenase

6. Cis-2-hydroxy-penta-2,4-dienoate hydratase

7. 2-hydroxy-cis,cis-muconate semialdehyde hydrolase

These are reported carbazole degrading enzymes which are produced by bacteria during its biodegradation. I need to know the protocols of the enzymes for confirming their pre(ab)sence. It will be great help if anyone can help me to get the enzyme assay protocols of at least of the first 4 enzymes.

Thanks in advance

Prasenjit Ghosh

PhD Scholar, IIT Bombay

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