Plasmid linearization for in vitro transcription?

Hello, We would like to in vitro generate mRNA from this plasmid, encoding SB100X transposase https://www.addgene.org/browse/sequence/178440/ using mMessage mMachine T7 Transcription Kit. We...

10 November 2019 9,943 3 View

The same conditions for conventional PCR could be used for qPCR?

I have a protocol for conventional PCR, the amplicon size is around 100bp and the number of cycles between 25-30. I supposed the temperature and time of each phase must be the same because I used...

17 December 2018 1,812 1 View

What is the dispersal /colonization capacity distance from certain passerines?

I am looking for reviews or articles which give distances for the following species: Anthus trivialis, Emberiza citrinella, Phoenicurus phoenicurus, Pyrrhula pyrrhula, Miliaria calandra, Galerida...

28 November 2016 302 6 View

I have previously nitrogen frozen tissue. Could I put it in OCT to make cross sectioning and immunohistochemistry possible?

01 November 2016 5,936 7 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View