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Quantification of CD8 positive cells using ImageJ software?

Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...

01 July 2024 8,964 1 View

Join writing on scopus, Can I join?

Good afternoon ladies and gentlemen. is there anyone who writes in the field of public administration and wants to write in scopus? I want to join.

30 September 2023 3,232 2 View

Plasmid linearization for in vitro transcription?

Hello, We would like to in vitro generate mRNA from this plasmid, encoding SB100X transposase https://www.addgene.org/browse/sequence/178440/ using mMessage mMachine T7 Transcription Kit. We...

10 November 2019 10,015 3 View

How to convert enzyme activity ratio 1:10000 to U/g?

I am trying to buy pepsin for an experiment (protein extraction from a certain type of meat), which requires me to make up a concentration of 6KU/g of meat. Having a look online I came across a...

10 April 2019 2,025 1 View

The same conditions for conventional PCR could be used for qPCR?

I have a protocol for conventional PCR, the amplicon size is around 100bp and the number of cycles between 25-30. I supposed the temperature and time of each phase must be the same because I used...

17 December 2018 1,896 1 View

Recommendations when working with kaolin-bentonite mixtures

Dear researchers, I am writing to ask you about your experiences when working with kaolin-bentonite mixtures in the laboratory. My idea is to perform some characterization and consolidation tests...

24 July 2017 246 3 View

Concept of apparent cohesion.

Dear all, Recently I was reading an article related to soft soil improvement by encased stone columns, subjected to lateral loading by means of shear tests. They mention that there is an increase...

12 July 2017 3,287 4 View

Why some chromosomes are delayed during meiosis?

I'm making a cytogenetic characterization of chromosomes in meiosis, specifically during prophase I and as you can see in the picture, this cell must be in pachytene forming a synaptonemal complex...

08 July 2017 5,984 5 View

What is the dispersal /colonization capacity distance from certain passerines?

I am looking for reviews or articles which give distances for the following species: Anthus trivialis, Emberiza citrinella, Phoenicurus phoenicurus, Pyrrhula pyrrhula, Miliaria calandra, Galerida...

28 November 2016 365 6 View

I have previously nitrogen frozen tissue. Could I put it in OCT to make cross sectioning and immunohistochemistry possible?

I have tissue that has been frozen in N2 without supporting medium (OCT). Could I put the tissue in OCT and put next in isopentane just to solidify the block and make the sectioning possible.

01 November 2016 6,016 7 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View