Plasmid linearization for in vitro transcription?

Hello, We would like to in vitro generate mRNA from this plasmid, encoding SB100X transposase https://www.addgene.org/browse/sequence/178440/ using mMessage mMachine T7 Transcription Kit. We...

10 November 2019 9,943 3 View

The same conditions for conventional PCR could be used for qPCR?

I have a protocol for conventional PCR, the amplicon size is around 100bp and the number of cycles between 25-30. I supposed the temperature and time of each phase must be the same because I used...

17 December 2018 1,812 1 View

Can anyone help me to download this paper?

Intra-Oral Hydrogen-Ion Concentrations Associated With Dental Caries Activity IntraOral Hydrogen-Ion Concentrations Associated With Dental... Thanks!!

23 September 2018 7,564 4 View

What is the dispersal /colonization capacity distance from certain passerines?

I am looking for reviews or articles which give distances for the following species: Anthus trivialis, Emberiza citrinella, Phoenicurus phoenicurus, Pyrrhula pyrrhula, Miliaria calandra, Galerida...

28 November 2016 302 6 View

I have previously nitrogen frozen tissue. Could I put it in OCT to make cross sectioning and immunohistochemistry possible?

01 November 2016 5,936 7 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

No title

01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View