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Quantification of CD8 positive cells using ImageJ software?

Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...

01 July 2024 8,964 1 View

Plasmid linearization for in vitro transcription?

Hello, We would like to in vitro generate mRNA from this plasmid, encoding SB100X transposase https://www.addgene.org/browse/sequence/178440/ using mMessage mMachine T7 Transcription Kit. We...

10 November 2019 10,015 3 View

How to convert enzyme activity ratio 1:10000 to U/g?

I am trying to buy pepsin for an experiment (protein extraction from a certain type of meat), which requires me to make up a concentration of 6KU/g of meat. Having a look online I came across a...

10 April 2019 2,025 1 View

The same conditions for conventional PCR could be used for qPCR?

I have a protocol for conventional PCR, the amplicon size is around 100bp and the number of cycles between 25-30. I supposed the temperature and time of each phase must be the same because I used...

17 December 2018 1,896 1 View

Can anyone help me to download this paper?

Intra-Oral Hydrogen-Ion Concentrations Associated With Dental Caries Activity IntraOral Hydrogen-Ion Concentrations Associated With Dental... Thanks!!

23 September 2018 7,631 4 View

Why some chromosomes are delayed during meiosis?

I'm making a cytogenetic characterization of chromosomes in meiosis, specifically during prophase I and as you can see in the picture, this cell must be in pachytene forming a synaptonemal complex...

08 July 2017 5,984 5 View

What is the dispersal /colonization capacity distance from certain passerines?

I am looking for reviews or articles which give distances for the following species: Anthus trivialis, Emberiza citrinella, Phoenicurus phoenicurus, Pyrrhula pyrrhula, Miliaria calandra, Galerida...

28 November 2016 365 6 View

I have previously nitrogen frozen tissue. Could I put it in OCT to make cross sectioning and immunohistochemistry possible?

I have tissue that has been frozen in N2 without supporting medium (OCT). Could I put the tissue in OCT and put next in isopentane just to solidify the block and make the sectioning possible.

01 November 2016 6,016 7 View

Sir can you tell me whether you deployed RFID readers in real time and collected dataset ?

Can you recommend me any open source tool in RFID to analyse the RFID dataset

01 January 1970 2,880 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View

PhD thesis topic?

I want to write a topic for my PhD thesis in hospitality (hotels), can u please suggest some variables

29 July 2024 9,058 3 View

What are the main types of errors and causes that English major college students commit in their English writing?

a 15-page paper

29 July 2024 1,956 5 View

I did hirshfeld surface analysis for molecule but did not find red spot and interaction surface in energy frame work, is it ok for research article ?

In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?

28 July 2024 2,988 0 View

How can I find writers for drafting, submitting, and publishing papers?

Looking for paper collaboration writers： Collaboration Model 1: 1.1 Based on the chosen topic, complete the paper writing, select an SCI or SSCI Q1 journal, submit using my QRCID, and complete...

27 July 2024 8,965 1 View

Given currenct advances in the AI and Smart Computation, what are the prospects of having truly Humanoid Computing Devices in support of Education?

Today, many priorities for improvements to teaching and learning are unmet. Educators seek technology-enhanced approaches addressing these priorities that would be safe, effective, and scalable....

26 July 2024 8,544 4 View

Could international experts in media and journalism, AI, communications and international communication share this conference link?

"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/

23 July 2024 3,139 6 View