I was doing a google search for the principle behind the amido black staining of total protein. However, I found information only about its usage in forensic sciences. I wanted to know how amido black specifically stains proteins.
Hi,
Amido black (as in 'amidoschwarz’ method of staining, developed by Schaffner and Weissmann in 1973) is an anionic dye. Its principle of action is described here:
https://books.google.bg/books?id=D9RwcUKQsHsC&pg=PA140&lpg=PA140&dq=amido+black+anionic+dye+protein&source=bl&ots=QiSysRTMdP&sig=ACfU3U0fg3UdOiFhY1VRnkZOhvcQ2uPgLw&hl=en&sa=X&ved=2ahUKEwjOr4mgvOHjAhW7w8QBHZ1xA7IQ6AEwCHoECAkQAQ#v=onepage&q=amido%20black%20anionic%20dye%20protein&f=false
Other relevant research:
Article A rapid, sensitive, and specific method for the determinatio...
(original article)
https://books.google.bg/books?id=nKlNDSnVOMAC&pg=PA167&lpg=PA167&dq=amido+black+anionic+dye+protein&source=bl&ots=yXsBHtsWY4&sig=ACfU3U3JAtowKv1yNMZmWHrg0CQxrd2pQQ&hl=en&sa=X&ved=2ahUKEwi_yNSpuuHjAhWFyqYKHVLgCl4Q6AEwB3oECAgQAQ
http://www.bio-rad.com/en-us/applications-technologies/total-protein-detection?ID=LUSQ3K15
http://europepmc.org/articles/PMC3932024/
I hope this helps :)
Hello Dr. Valeria,
Thank you so much for your prompt response. From what I understood, amido black being an anionic dye binds to the cationic residues of the basic amino acid residues.
...binds cationic residues of the basic amino acid residues and the free amino terminal group of the protein.
If you require further information, you can find it in this book:
Walker, J.M. (ed). "The Protein Protocols Handbook, 3rd ed." New Jersey: Humana Press, 2009.
I hope this helps :)
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