Hi
I am looking for a new and step-by-step method for extracting sage silymarin from seed, vegetative organ and callus.
Can anyone help me?
[email protected]
1- Collection the required part of the plant.
2- Wash with tap water
3- Dry under shade.
4- Grounding ( make it powder)
5- Extract with different solvent, starting from non-polar ( hexane, ethyl acetate,
methanol and 70% methanol.
6- First extract ( hexane is oils and oily compounds).
7- Take TLC for next two extracts, to know the number of components.
8- Evaporate the solvents by rotary evaporator,
Next step is related to What you want ( single pure compounds or used as extract.
9- Separate pure single compounds using chromatography ( the best one is MPLC
flash chrom.) or column chrom.
10- Evaporate the solvents by rotary evaporator.
11- Apply the separated compounds according to your need.
Check https://www.tandfonline.com/doi/pdf/10.1080/08327823.2017.1320265?needAccess=true
Mam, you can also try with sequential ombinations of extraction using Soxhlet, microwave, ultrasonic, supercritical, or maceration methods to ensure complete extraction.
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