They are living (M1, M2 and M3) and also lugol-fixed (M4 and M5) and belongs to a reservoir.
Thanks in advance
Hi Maria!
It seems to be Oocystis sp. or Gloeocystis sp.
Oocystis sp.: Planktonic; colony of 2-8 cells surrounded by cell wall of their mother cell, but sometimes unicellular; cell body broad ellipsoidal, both ends slightly pointed and with a thick cell wall; 1-3 chloroplasts parietal plate-like, with a single pyrenoid...
Gloeocystis sp.: Planktonic or benthic, Colonies spherical or aggregates in shape; sheath conspicuous; cell body spherical in shape; chloroplasts cup-shaped or plate-like...
best wishes
Bohuslav
first three may be some species of Oocystis. But the brown colored seems to be cyanobacterium such as Chroococcus or Gloeocapsa as the semicells are tapered at edges.
The colony and cell wall structure certainly look like Oocystis, but the contents of the cell doesn't. It seems to be very homegenous, Oocystis is different, see picture. I use Komárek and Fott 1983 for identification of this group and it does not encourage to use the genus Gloeocystis.
Thanks for your answers (the brown colour is not real, they are fixed with lugol!!)
I was also using Komarek 1983, I was thinking in some Genus like Neglectella, Oonephris, Eremosphaera, ........... or Glaucocystis
But the top three ones are not.
Your other suggestions could be correct, I have no experience with these genera so I'm afraid I can't help you there.
I believe these 2-celled colonial greens could be identified as either Oocystis or Glaucocystis. They are also defined by their ellipsoid cells that are enclosed within the cell wall of an enlarged “mother cell”. Yet, it is difficult to tell if the plastids in these photographs are green or bluegreen. According to P. Bourrelly, Les Algues d’eau, Algues Vertes (1966), if the plastids are green it keys out to Oocystis. If they are bluegreen it’s Glaucocystis. I would guess Glaucocystis nostochinearum.
Dear maria
Your photographs are awesome .. which microscope and camera you are using...
With regards..
Perumal
Dear Perumal
I use a Nikon Eclipse Ti microscope (inverted microscope) with a Nikon digital sight DS-Fi2 video camera with a NIS_ELements Program (analysis image program).
Best regards
Maria
Maybe it's a species of Oocystis, but chloroplasts are similar to those of Eremosphaera. See E. tanganyika.
First green colour with ellipsoidal cells and the brownish tinge round cell may be different algae because the cell shape is quite different. This brown one can be chroococcus species but I am not sure about the first whetherr oocystis or the other one
Which one is Gloeocapsa? Or you many all in their different phases?
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