Hi, 

Your question is way too generic.

Here a few things you might consider before asking:

- What is the target compound/s (from chemical classification down to specific moieties of interest)

- What is the matrix (blood, plasma, tissue, specific organ or part of it, cells, bacteria whatever..)

- Has anyone done it before? What is the state of the art? (search with whatever tool you want).

When you have answers to these 3 questions, build a first draft for your method. Then you might consider asking around for help.

But the question you ask is so generic that no one can really give you an answer.

I fully agree with the above.