Fluorescent tyramide protocol ?

Hello, Im asking to check if anyone has a protocol for preparing fluorescent tyramide for fluorescent in situ hybridization. The one Im using isnt working, so if anyone has one that works, can you...

05 November 2020 9,566 3 View

How can I fix our rodent's treadmill?

Our rodent'treadmill's bars show voltage but not affected animals. I cleaned all those bars as rat's urine is dielectric. Do you have any idea that helps me? Thanks

20 September 2020 5,971 2 View

Assessment of the exercise intensity?

Hi, How can I assess the intensity of a treadmill exercise in rats without measuring Vo2max? I mean how can I know a specific protocol has a low or high intensity without measuring any factors...

01 September 2020 3,788 5 View

Hi all, I would like to know the intensity of these two treadmill protocol are the same in Rats?

I would like to know in two different treadmill protocols, if we are going to reduce the treadmill velocity for rodents (14 m/min instead of 20 m/min) but increase the time of exercise (60 min...

23 August 2020 350 2 View

What is the talents' best identification tool?

There are different identification tools to identify talented individuals, which tool you think is the most successful one to properly identify them?

04 October 2019 5,734 3 View

Problem in Wi Fi connection ?

I have a problem with the modem (D-link). When you set up the modem, I connect normally, but after a few time, the Wi-Fi network is disconnected and when I try to re-enter in the network, it does...

03 September 2019 8,187 4 View

What's the simplest method to determine the HOMO and LUMO energy levels of insoluble organic semiconductors?

HOMO and LUMO energy levels determination is un important step to confirme organic materials application in OSCs

19 February 2019 1,327 5 View

According to SJR, there are a Cataloging and Classification Quarterly (Q1, Q2, Q3 and Q4). I would like to know the Q1 and Q2 of psychology journals?

Dear Colleagues, According to SJR, there are a Cataloging and Classification Quarterly (Q1, Q2, Q3 and Q4). I would like to know the Q1 and Q2 of psychology journals? Thanks a lot

08 September 2018 8,737 1 View

No title

24 May 2017 3,415 18 View

Colony PCR, Product, recombinant Colony?

02 January 2017 6,959 8 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View