Can anyone help in progamming the protocol for IEF-cell bio-rad of 11cm and pH 3-10 strip?

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Dear's, Is it possible to separate the Lysine proteins through electrophoresis?

how we can separate the lysine proteins through SDS-PAGE and their identification in electrophoresis.

09 October 2017 6,059 6 View

What would be the possible reason for steeking of proteins in 2D Gel Electrophoresis?

after running the 2nd dimension of gel electrophoresis proteins are constructed in a steek line and they do not get separated.

07 August 2017 932 0 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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01 August 2024 4,673 4 View

How to check pH of a high strength hydrogel?

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EDTA buffer solution?

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Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

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29 July 2024 8,560 0 View

Why my gel electrophoresis has smearing?

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How should we increase the quality of RNA extraction?

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22 July 2024 155 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

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19 July 2024 148 6 View

Less substrate signals towards the last lanes of the Native PAGE?

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Ronald Van Vlierberghe

Hi Aadil,

I always used the following steps:

1. 12h passive rehydration

2. 20 minutes at 250V, linear

3. 2.5h at 8000V linear (going from 250V to 8000V)

4. Up to 30,000Vh rapid (Stay at 8000V to a total of 30,000Vh)

With kind regards,

Ronald

Benjamin THOHA Thomas

Good submission by Ronald

Aadil Tantray

Thanks, Ronald