Can anyone have an experience to measure Xanthine oxidase activity in macrophages or any other cells?

More Naveen Kumar Tangudu's questions See All

Can anyone explain how to calculate encapsulation efficiency(Plant extract encapsulated alginate nano particles) using total polyphenol content?

Please suggest me if there are any reference materials for this topic. Thank You

30 December 2020 3,799 2 View

Electric field inside a biased N-type uniformly doped semiconductor?

What does the profile of electric field look like in a uniformly doped N-type semiconductor when an external voltage of 'V' volt is applied? Please provide any reference material if possible.

06 November 2020 1,987 8 View

Plane stress or plane strain for Railway Ballast system ?

Dear All, I am working on a wooden rail sleeper analysis. Using the Druker Prager model for Ballast and soils, Initially, I assumed the 2D Behavior of RailSleeper and Rails was Plane Stress and...

04 October 2020 5,459 8 View

What should be Optimum Review duration for a research/review article for any [unpaid/freely publishing]journal till acceptance?

Dear Research Gate Members, We all have experience regarding publications of research/review articles in various journals. Some of the journals review the articles in 4-6 weeks and some takes...

01 October 2020 7,201 1 View

How to write an impressive review paper in the field of Industrial effluent treatments?

I am currently working on collecting literature based on the available treatment technologies for industrial effluents. There are vast variety of methods physical, biological and Advanced...

23 September 2020 2,314 4 View

Fibrous rock with triclinic symmetry?

I am working on wave propagation in fiber reinforced material. Please provide me an example of rock-type which is consisting property of fiber and exhibiting triclinic symmetry with its elastic...

20 September 2020 9,874 1 View

How to pursue modelling and map Traditional Knowledge?

I was wondering is it possible to map Traditional Knowledge (TK) of a bigger region (say staus of TK) on the basis of sampling in a smaller region through Arc GIS and modelling? Say we sampled in...

13 August 2020 308 3 View

Pressure behavior from CFD simulation and testing?

Hello, I am simulating gas flow from a high-pressure (700 bar) to a low-pressure container as shown in the attached figure. The red curve shows the pressure curve obtained from the testing, while...

04 July 2020 2,077 2 View

Ansys Fluent remeshing on hex mesh?

Hello, I went through ansys fluent dynamic meshing guide. It says remeshing can be done only on tetrahedral mesh. I still tried to implement it on hexahedralmesh. It works well. It is bit...

03 May 2020 2,677 4 View

How AI and Deep Learning can help to Screening Corona virus (COVID-19) ???

There are many Articles out there related with Deep Learning with corona virus, For Example : "deep learning algorithm using CT images to screen for Corona Virus Disease (COVID-19), Deep Learning...

12 March 2020 3,780 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Can anyone help me with doing resorption pit assay on biomaterials for 28 days?

I culture RAW 264.7 cells on my samples and differentiate them to osteoclasts with RANKL. I want to do resorption pit assay by culturing cells for 28 days. My problem is that the macrophages keep...

01 February 2021 6,746 2 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

What are the validation parameters for RAW BLUE macrophages 264.7?

Currently, I am working with RAW BLUE cell lines to check the SEAP expression on addition of TLR ligands? Method developed for different TLR ligands. How experiments can design for validations?...

24 January 2021 589 2 View

How to assess monoamine oxidase levels ?

hello everyone i have a research about monoamine oxidase enzyme and i saw a lot of detection method of it including monoamine oxidase activity assay kit but i don't know what is the easiest and...

18 January 2021 3,474 2 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

Please what is the best solvent to dissolve heme?

I have tried NaOH and NH3 so far but can't dissolve well.Thanks for the assistance. I can be contact personally on this email:[email protected]

04 January 2021 5,528 2 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

Awatif Hameed Issa

Dr. Naveen

Look to this Ref. may help you

Beckman, J. S., Parks, D. A., Pearson, J. D., Marshall, P. A. & Freeman, B. A. A sensitive fluorometric assay for measuring xanthine dehydrogenase and oxidase in tissues. Free Radic. Biol. Med. 6, 607–615 (1989) .

Naveen Kumar Tangudu

Thanks Awatif for your reference.. however we tried sigma kit.. which worked well..