Can we use RNA-seq library for gene expression studies?

Since our RNA is less abundant, can we use the libraries prepared for RNA-seq to validate (qPCR)the results of RNA-seq.

12 November 2020 803 6 View

For primer efficiency calculation can we use positive control(in which gene is abundantly expressed) and perform the analysis?

My sample of interest is sperm RNA(less abundant and highly fragmented RNA). In that case, to determine the primer efficiency, can i use tissue samples (in which the gene is abundantly expressed)...

11 November 2020 758 3 View

What is the difference between semantic role labelling and named entity recognition?

Natural language understanding

04 May 2019 7,442 9 View

How to calculate network performance using ns3?

I'm new to this network domain. But i want to learn this area thoroughly. So I'm currently dealing this subject. I'm learning ns-3 simulator. Please help me to know how to analyze the performance...

17 September 2018 3,246 3 View

Acyliminium reactivity versus enecarbamate?

Dear researchers, i kindly ask you to help me with the following aspect: in scheme 4 (of j.org. chem, vol.66,No.23, 2001) it is shown that nucleophile attacks on the acyliminium intermediate and...

08 February 2016 1,601 3 View

I am using HEC RAS to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable analysis?

I am using HEC RAS (Though it is a river analysis software) to an urban area to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable...

01 February 2016 4,321 6 View

Why does K estimator fail to estimate Ka and Ks values for certain gene combinations?

12 February 2015 8,709 1 View

Can any one explain me the pixel recursion and how to calculate new disparity candidate using this?

Pixel recursion.

11 August 2014 2,495 2 View

Why is a logarithmic current amplifier used instead of a linear one in scanning spreading resistance microscopy?

For measuring and amplifying current during a C-AFM scan, I'm aware that a linear current amplifier is generally used but in SSRM which is mostly used to get doping profiles in semiconductors, a...

11 April 2014 4,026 3 View

What is the exact definition for entropy or what does entropy really mean?

Entropy is defined relatively with temperature and enthalpy of the system but what type of characteristic is entropy?

20 February 2014 5,501 9 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View