29 September 2020 1 6K Report

Are there some comparative country studies about civilian oversight of the police, especially for CEE countries?

More Teo Giljevic's questions See All
Can there be two dependable variables in a model?

I am just wondering if anyone has seen a two dependable variables in a model? Love to hear what people have to say!

02 May 2024 9,456 2 View

Good day, have you or ever read any articles related to the ripple effects and vicarious reinforcement in students' learning process?

Good day dear researchers, Please share articles related to the ripple effect and vicarious reinforcement in the students' learning process if you have read them before (only the article title...

06 July 2023 4,523 6 View

Which method to use for dealing with missing data (MCAR)?

My study has the following details: N=130 3 Conditions 5 Timepoints 6 Variables (measured at every time point) Analysing using Little's MCAR test revealed that my data is missing completely at...

29 April 2023 3,848 0 View

How to prevent cells from dislodging from well plates for when changing media?

I am having trouble with C2C12 cells detaching from the well plates after adding conditioned media. Areas on the left and bottom sides of the wells lack cells after switching to the conditioned...

24 October 2022 9,802 6 View

How to prepare 10mM acetic acid and 20% ethanol?

I am trying to dilute the TGF-B3 growth factor with the recommended diluent: sterile-filtered liquid containing 10mM acetic acid and 20% ethanol. My questions: 1. How to prepare 10mM acetic...

16 May 2021 9,323 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,795 3 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 8,190 3 View

Anyone with experience in culturing Normal Human Astrocyte?? Are these cells healthy?

Dear All, I am new to this Normal Human Astrocyte (LONZA). They are primary culture. The first vial of cryopreserved cells that I revived seems ok, however, another does not. I noticed a big clump...

22 January 2021 4,750 2 View

Can qPCR used to quantify cells over other methods like ICC & flow cytometry?

I am currently working to generate a certain type of neurons. However not all the cells in the culture will be expressing the specific markers. I would like to check how many cells are expressing...

03 October 2020 5,019 2 View

Abnormal morphology of SNL76/7 fibroblasts of my culture, what has been wrong to them?

As I know SNL76/7 is a passage-independent feeder, hence I assume they will not change morphologically and karyotypically, after indefinite sub-passaging. However, recently I noted the changes in...

09 August 2020 9,769 6 View

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