I am looking for agents that definitely bind alpha synuclein (fibrils), and could potentially be displaced by novel compounds, inorder to set up an in vitro assay for alpha synuclein. So far I have come across Thioflavin T fluorescence assay (plenty of references) and a [3H] Chrysamine G radiometric assay. I have not been able to find much about the latter. Does anyone know of any suppliers of [3H] Chrysamine G? Any one with experience of this assay?
Hi Nisha, I'm afraid that Chrysamine is not specific for synuclein. It also binds abeta peptides.
I have a paper on using common commercial fluorescent DNA probes to do studies on amyloids such as abeta peptides and do competition studies (attached).
I found that these probes bind abeta, prion and amylin peptides. I would be thrilled if you tried these probes on a-synuclein and saw them bind.
Article Common Benzothiazole and Benzoxazole Fluorescent DNA Interca...
Thank you very much for the paper, Steingrimur Stefansson. I will read up and see if it is suitable for our purpose.
Regards,
Nisha
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