Need some help to make sense of contact maps generated by gmx mdmat. Could anyone help?

I used gmx mdmat in gromacs to create contact maps, but I am unable to make sense of the outputs I've got from the same. Could anyone point me in the right direction? This is an attempt to...

05 April 2020 2,189 3 View

What solvents can help make a PLA gel stable at room temperature?

What solvents can help PLA form a gel in the solution at room temperature? Although we can dissolve PLA in solvents by heating above Tg, I wish to know if there is a possibility that the solution...

16 February 2020 2,691 2 View

Graphs in ITC experiment show binding but doesn't fit any typical standard reaction graphs. Could anyone help??

Dear All, I have performed an ITC experiment with a membrane protein and its lipid substrate. I have got graphs which are a bit confusing since it is quite unlike any other which is available...

10 February 2020 8,143 4 View

Should I need to use a glove-box to fabricate a lithium ion battery based on water-in-salt electrolyte?

I was wondering if the fabrication of lithium ion batteries based on water-in salt electrolyte could be performed in the ambient atmosphere. Can anybody help me with this?

06 May 2019 8,020 3 View

How to obtain per base read coverage of a bam file in excel format?

I have sequenced a 7 kb part of frog mtDNA and have generated a bam file of the sequence. In IVG, I can visualize manually the per base read coverage but I need this information in an excel format...

09 June 2018 9,444 2 View

How to obtain information as a text file from NGS pileup data in IGV?

I have generated a pileup of Illumina Miseq NGS data in IGV by mapping the reads onto the reference sequence reconstructed from the reads in order to check how well the sequencing has worked. The...

22 October 2017 9,936 3 View

Why don't single strands anneal among themselves during annealing step of PCR?

At the annealing step of PCR it is the oligonucleotide primers that binds to the single stranded template and initiates extension. But do the two single strands also anneal among themselves?

17 June 2016 4,322 3 View

What are the disadvantages of designing a very long primer (50 bases>)?

I am trying to design primers for amplifying certain genes across the amphibian taxa. I know that the lower limit for designing primers is 18 bases for reasons of specificity. However, what could...

01 May 2016 1,563 5 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View