Debjyoti Boral

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Questions related from Debjyoti Boral

How to procure substrate PMA (para methoxy 2 amino benzamidoxime) for enzyme analysis?

I want to procure the substrate PMA (para methoxy 2 amino benzamidoxime) for my enzymatic analysis. Please suggest a make or any relevant contact for the same.. There's only one Cayman Chemicals....

19 July 2024 6,012 1 View

How to prevent or reduce the leaky expression of only GST in a GST fusion protein?

We're getting an intense 26 kD band along with our band of interest using pGEX4TI vector. Any suggestions on how this intense band can be removed or at least reduced? We're using BL21 Codon+ for...

31 May 2021 5,302 1 View

Need some help to make sense of contact maps generated by gmx mdmat. Could anyone help?

I used gmx mdmat in gromacs to create contact maps, but I am unable to make sense of the outputs I've got from the same. Could anyone point me in the right direction? This is an attempt to...

06 April 2020 2,296 3 View

Graphs in ITC experiment show binding but doesn't fit any typical standard reaction graphs. Could anyone help??

Dear All, I have performed an ITC experiment with a membrane protein and its lipid substrate. I have got graphs which are a bit confusing since it is quite unlike any other which is available...

11 February 2020 8,249 4 View

Can anyone give me any information about labs who synthesize intermediate lipid substrates in the membrane biosynthesis pathway?

I need substrates for an enzyme involved in the pathway. So any help would be appreciated.

07 August 2018 7,518 0 View