Are transcripts with class code X could possibly be the anti-sense RNA transcripts?

We prepared un-stranded RNA-Seq library (SMART-Seq2) and sequenced in Illumina platform. We mapped with STAR (by adding XS tag) and assembled using cufflinks RABT assembly. Now after comparing...

11 December 2018 2,352 1 View

What are the essential SNP filtering criteria one should follow before performing GWAS analysis?

We are working on a project where we need to perform genome wide association studies for multi-parental population. We have the SNP data from whole genome re-sequencing protocol. As I am quite...

09 October 2017 448 9 View

What are the considerations for choosing a statistical model to perform association studies?

07 August 2017 4,004 5 View

How to analyze BCFTools RoH output? How to provide information of in-house alternate allele frequency?

10 November 2016 571 1 View

Can anybody help me in understanding one variant reported in 1000Genomes phase three release?

11 December 2014 3,740 5 View

Is there any freely available tool to predict network from vcf file?

Rare complex disorders believed to be caused by combinatorial effect of many genes. Our lab works on one such disease. We are done with WES of four affected members from such a family. We are now...

07 August 2014 8,736 4 View

What if anyone removes all the unknown (or confusing) contigs from the reference genome (eg hg19) before alignment and other consecutive tasks?

There are a good number of contigs in the hg19 release. The coordinates of this contigs are a bit confusing. Now if anybody want to keep these contigs out of reference before doing alignment, what...

01 February 2014 7,190 3 View

Which variant caller researchers are using frequently?

I am developing/refining a pipeline for exome data. According to reported articles there are mainly two callers I found out to be more acceptable among researchers: One is SAMTools' variant caller...

10 November 2013 782 5 View

Is there any repositories where I can find a vcf file for dbSNP release 131?

I am looking for a vcf file of dbSNP release 131. Can anyone provide me with a link holding the data? Or is it possible to convert dbSNP flat files to vcf format by using any tool?

10 November 2013 7,781 7 View

How to correct SAM file for the errors reported by PICARD ValidateSAMFile ?

I am trying to validate SAM file for further downstream processing using picard ValidateSAMFile command. But getting four types of error for ~50000 reads. Summary coming as: Error Type...

09 October 2013 5,409 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View