Can anyone give me a working protocol to extract DNA from Salmonella colonies using chloroform-isoamylalcohol (24:1)?

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What can I change if I'm not getting colonies with my Gibson assembly product?

I'm assembling one PCR fragment (4.2 kb) with my vector, pECFP (4.8 kb), and transforming them into NEB 5-alpha Competent E. coli cells. Both the fragment and the vector were purified using the...

04 March 2024 1,090 5 View

Which solvent is better for acetic acid extraction from water?

Hello everyone, I have a question about properties of 2 different solvents. For little context, I have a homework about liquid-liquid extraction so I had to compare which solvent is better for the...

16 November 2023 4,662 1 View

Mitotracker and lysotracker in neurons?

Hello. Does anyone have any experience and tips on producing quality lysotracker and mitotracker stains in neurons? I can get a signal for both, but they're quite inconsistent-- I often get a...

26 February 2023 2,737 1 View

For PCR products, why would bands show on a 1% TBE buffer gel, but not on a 1% TAE buffer gel? Same PCR products, different gels, but why?

Hello all, I'm trying to use electrophoresis to separate 3 operons that are 500-600bp, for gel extraction and direct sequencing. When I check my PCR products on a 1% agarose TBE gel, I have clear...

04 December 2022 8,209 7 View

Electrical behavior of ICPs (intrinsically conductive polymers), ECAs (electrically conductive adhesives) and inorganic semiconductors?

Hello everyone, first I will like to get ahead of the jargon-confusion with all these abbreviations. Intrinsically conductive polymers (ICPs) also called conjugated polymers, doped polymers or...

07 September 2022 3,900 0 View

What happened with my DNA extraction?

Hi, I was trying to extract DNA with TRIzol. When I added chloroform to my sample with TRIzol and after centrifugate it, my phases were inverted. (Photo below) -De antemano muchas gracias

29 August 2022 2,553 4 View

Sanyo Incubator Compatible Humidifying Pan?

Hi there! I've been looking everywhere for a compatible humidifying pan for our Sanyo MCO-18AIC incubator to no avail. Does anyone have any suggestions for which pan might be compatible with this...

05 July 2022 2,497 0 View

How to calculate the energy efficiency of hydrogen production process by gasification from biomass with the Aspen plus software?

How can calculate the energy efficiency of a hydrogen produced from agricultural residual biomass gasification process in Aspen plus software?

31 March 2022 4,113 3 View

What are the cell levels (cells per mL or uL of blood) for haematopoietic progenitors in the murine circulation?

Dear all, I am trying to use the Fortessa flow cytometer to investigate the cell numbers of several populations of the haematopoietic tree (LT-HSCs, LSK, CMP, CLP, GMP and MEP) in the bone marrow...

18 October 2021 9,382 0 View

Does anybody could give us some insights or suggestions?

My co-author and I are working on a paper using the Economic Freedom of the World and 19 countries (sample cannot be increased because it is focused on a specific Region). The number of years is...

18 August 2021 1,274 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View