What are the SPSS commands to calculate item discrimination index and difficulty index as part of item analysis?

Hi everyone! I am trying to find the "how to" on item difficulty and discrimination on SPSS and its interpretation. I have read about and performed the command analyze> scale> reliability...

21 September 2020 4,040 3 View

Does somebody know about an index that measures Populism among countries?

I'm looking for something similar to the Economic Freedom of the World Index but measuring Populism... I do not know if it exists. Thank you

22 June 2020 4,460 8 View

Does anyone have a protocol to do nuclear extraction for enzymatic activity assay?

I would like to measure enzymatic activity of some nuclear proteins, but I have troubles with the extraction and keeping its native form. Sonication seems to be a must in my cells to release...

23 September 2019 7,472 7 View

How to estimate and plot marginal effects form an interaction model with data panel in Eviews 10.0...?

Who knows how can I estimate and graph the marginal effects from an interaction model -regression data panel- in EViews10.0. Y = X + Z + XZ I need to graph the marginal effect of X on Y...

07 April 2019 2,013 1 View

How to use molecular sieves to dry Methanol or Ethanol?

So i'm trying to use molecular sieves to dry methanol, but i can't find a good tutorial on how to do it, any help would be greatly appreciated.

10 February 2019 299 3 View

How I quantify DNA in GENESYS 10S UV-Visible and Visible spectrophotomete?

Hi, I want to quantify DNA, I have a GENESYS 10S UV-Visible and Visible spectrophotometer, but I do not have the instructions. Could someone tell me what to do? Do I need some software? Thank you

10 August 2018 6,181 2 View

Which has been the major contribution of the mechanical engineering to the development of renewable energies?

Contributions of the mechanical energy to the renewable energies (Utilization, discovery, creation, etc)

08 April 2018 8,984 5 View

Whats the recommended volume for 6-well plates in cell culture?

Hi, I am new to primary cell culture and I'll be working with chicken-derived dendritic cells. I'll be using GM-CSF and IL-4 to induce their maturation in 6-well plates with RPMI-1640 media. I...

05 February 2018 3,940 4 View

Does anyone know where can I buy chicken CD40 antibody for FACS?

Hi, I'm working on a project where the main objective is to measure the maturation markers of chicken-derived dendritic cells. Naturally, I am looking at CD40, CD80/86 and MHCII markers via flow...

05 February 2018 2,475 3 View

In Cancer Epidemiology, which indicator should be more suitable as estimator of burden of disease?

Dear RG community: I am currently working on an ecological study in order to analyze disease burden from cancer in my country. I am looking for the most suitable indicator of disease burden....

23 January 2018 8,059 11 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View