CTLA4 expression by Treg cells

Hi, I'm working in the detection of CTLA4 on human Treg cells. Our assay measures intracellular expression of CD152 by fow cytometry, in CD4+/CD25hi/CD127lo T cells, in a 20 hour stimulation...

06 July 2017 6,781 2 View

Is affinity purification of antibodies to a hapten possible?

I have been trying to purify by affinity chromatography antibodies to small peptides covalently linked to BSA with no success. Recovered antibodies completely lost activity. Now I'm interested in...

02 March 2015 4,042 12 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

Is there such a thing as Igg specific MHC class II epitopes or do all non-self MHC class II epitopes trigger antibody production of all subtypes?

I know there are bioinformatic tools that allow epitope prediction to determine if a certain peptide would trigger an immune response. I also read that for allergen diagnoses IgE of sensitised...

09 February 2021 4,173 3 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View

Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

How to measure the relationship between students' readiness to E-learning and their academic performance?

I'm currently studying the relationship between students' readiness to E-learning and their academic performance. The variables are: Students' readiness: I intend to measure it through...

03 February 2021 9,207 8 View

What is the success rate of the e-learning plan in achieving the objectives of the curricula?

I need to know the reliability of the evaluation results and the rate of achievement of the course objectives, Thank you (*-*).

02 February 2021 7,828 7 View

What is the success rate of the e-learning program in your country during the time of Covid-19?

I need to know the extent of the success of the e-learning plan in countries of different capabilities, Thank you (*-*)

02 February 2021 4,712 15 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

What is the difference between Online Teaching and Emergency Remote Teaching in terms of effectiveness?

Due to the pandemic crisis, institutions opted for an unplanned online teaching for the safety of its students. While some believe that emergency remote teaching is as effective as other types of...

26 January 2021 8,421 21 View