Francisco Manuel Marco

3 Questions 7 Answers 0 Followers

Questions related from Francisco Manuel Marco

CTLA4 expression by Treg cells

Hi, I'm working in the detection of CTLA4 on human Treg cells. Our assay measures intracellular expression of CD152 by fow cytometry, in CD4+/CD25hi/CD127lo T cells, in a 20 hour stimulation...

07 July 2017 6,858 2 View

Is affinity purification of antibodies to a hapten possible?

I have been trying to purify by affinity chromatography antibodies to small peptides covalently linked to BSA with no success. Recovered antibodies completely lost activity. Now I'm interested in...

03 March 2015 4,098 12 View

Can anyone give me a reference on detection (or detectability) of allergen Der p1 by IgE immunoblot?

Some time ago I heard that Group 1 allergens like Der p1 are not detected on IgE immunoblots, but I can't find a publication describing it.

01 January 2015 9,562 4 View