Currently i'm doing research on thrombosis inhibition and already got the EC50 of my extract also EC50 of commercial drug. The extract is still crude, and the other hand the drug is single compound.
Please, make clear in your question. What is extract, what is drug and what is compound?
The lowest EC50 causing thrombosis inhibition is the potent conc.
The extract is methanolic extract of a plant. (Plant powder was macerated using methanol, filtered, than the filtrate vaporized into dryness).
The commercial drug was ticagrelor.
We got ec50 for exract and ticagrelor about 100ppm, and 0.6 uM respectively.
Can my extract cosidered as potent?
Dear Tri Wiyono,
You have an extract, i.e. mixture of different methanol soluble compounds. You do not know exact molar mass of your compound to convert it to uM for comparing. If you ask, is my extract potent than ticagrelor, it is not, because your compounds conc. is much higher than the drug. You should separate your extract for individual compounds and see their own activity. Then you can compare their activity with exact conc. of your compound. I hope for your success. Good luck!
Thank you.
Indeed, we need this consideration for additional argument so that this extract need to be isolated or not. Because for previous research this extract is polar and rich of polyphenols (i.e tannin).
We think tannin separation is a great chalenging job.
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts