can Tris buffer interfere in any way in a NaBH4 trapping assay that is carried out to assess the dRPase activity of a protein? A 5' dRP moeity may react with amine grps in a protein to form a schiffs base. This can be reduced by NaBH4 to form a DNA-protein complex. The reaction buffer will have HEPES as the buffering agent, but the recombinant protein that I am using to assay the 5' dRPase actiivty is stored in a Tris buffer. will this interfere with my assay?
Afreen Haider
Thanks for your reply Anastasiya! I was not able to find any report where Tris was used instead of HEPES. It is a relief that you get positive results with tris.
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Daniel Lerda
Dear Afreen Haider
Tris buffer in which the protein is stored not interfere with the assay. Best regards
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