Can anyone explain why in the initial denaturation of the TAIL-PCR most of the time we use two temperatures (for example 93 and 95 °C)?

10 October 2013 1 7K Report

Is it possible to use one temperature like usual PCR (95 °C for 5 min)?

Probably because that was what was first reported, and has been followed since. Most likely, you could just do the 95C step for five minutes and achieve the same results. Try it and see.

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Science method

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