Hi,

I was checking pDNA-Polymer binding affinity in Gel electrophoresis.? After running gel, I noticed complete migration of pDNA as well polymer -pDNA complexes. (Even in higher N/P ratios - 10,20) . I used Agarose 0.6% , 80V , 60 min.

Again, I gradually increased Agarose concertation , 0.8, 1, and 1.2% and voltage 80 to 100 V) - same problem- Complete migration.

Then, I increased pDNA concentration from 10 ng/microliter to 50 ng per microliter. Same issues.

I attached my Gel picture for reference

I performed PicoGreen Assay - it showing good binding affinity, but while running gel , I am not able to see polymer pDNA binding.

Can anyone give some suggestions to troubleshoot this problem.

Thank you