Ram Prasad Sekar

3 Questions 9 Answers 0 Followers

Questions related from Ram Prasad Sekar

Can anyone explain what is the reason for complete migration of pDNA-Polyplexes in Agarose Gel Electrophoresis ?

Hi, I was checking pDNA-Polymer binding affinity in Gel electrophoresis.? After running gel, I noticed complete migration of pDNA as well polymer -pDNA complexes. (Even in higher N/P ratios -...

04 February 2025 9,088 3 View

How to conjugate/tag FITC with polymer containing hydroxyl groups ?

Hi, I need to tag FITC with my polymer containing hydroxyl groups. The polymer is highly hydrophilic. I saw so many literature for FITC coupling with amine groups. Very few reports on...

20 October 2021 1,124 4 View

How can I select a solvent system for highly polar compounds to elute in TLC and run column?

I linked amine functional to Polar compound that contains acid group. I need to purify the compound but it is not soluble in organic solvents and I tried TLC (10,20, 50% Ethylaceate/hexane, 5%...

11 November 2015 8,274 11 View