when dissolve in solvent, if it give on 290 and when loaded in lipid nano particles it give 280+ not stable. is there any problem in my baseline calibration?
Firstly, chech the baseline of calibration with same solvent that use to desolve the drug and the change in uv value it is normal happens as doner-acceptor transter reaction to induse adutive rlecteostatic bond
If we disolv same drug in diferent solvent. It also show litle shift , why? Becouz of its conversion into derivativs???or another reasn?? Need ur sugession.thnks