Can anyone detail me about how to quantify TAGs using TLC ?

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deleted the question.?

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Thijs Kops

have you separated the lipids from the other compounds? If so extraction of the lipid dots from the TLC plate and measuring those after methanolysis on GC-FID or MS could work. directly on TLC it is very difficult to quantify any compound accurate.

Sk Riyazat Khadim

Thank you Thijs Kops and Simon Hammann. I have extracted the lipids using a modified Bligh and Dyer Method. I want to know if derivatising TLC separated TAGs and subsequent analysis via GC-FID or MS would render me the exact quantity, because trans-esterification would cause each lipid attached to glycerol backbone in a TAG molecule to be converted to respective FAME, then how there will be any existance of TAG molecule after derivatization. Please feel free to discuss, I might be missing something here.

With best regards