Both seeds are recalcitrant and have relatively small, thin cotyledons that do not appear swollen, so I assume that that the surrounding tissue is endosperm but cannot be certain.
Both Litchi and Strychnos have endospermic seeds. Please check the PDF attachments.
I am planning to conduct a study with the following variables: A - independent variable B - intervening variable C - dependent variable D - moderating variable between B and C The framework...
22 October 2020 5,324 3 View
Hello everyone! I am working on some feedback, and someone suggested that I run a replicated regression on my data (semi-quantified western blots mostly) instead of an ANOVA with a Dunnett...
04 August 2020 1,052 3 View
I am testing biofilm samples from the tongue for universal 16s rDNA using Sybr Green qPCR. The primer set worked well for two years, but all of a sudden we are not getting proper amplification,...
28 August 2019 6,465 2 View
We have a brown fluffy fungus growing on plant material samples and we are not sure what it is. It also does not grow on a petri dish either so we are not able to isolate it. Is there a way to...
04 June 2019 3,105 2 View
I am doing my thesis with a total of 206 samples. I am a bit confused about what software should I use to analyse the data; either SPSS / SEM? And why should I use that? My lecturer suggests...
08 May 2019 1,840 4 View
Hi all, I'm looking for a non-toxic UV reflecting white paint or alternative to paint a range of clay model morphs of moths that I will put in the field to record survival statistics. The models...
26 February 2019 8,902 1 View
Teaching and learning is heavily influenced by Information Communication and Technologies (ICTs). Students have become inseparable with their handheld devices and the most common of them is the...
04 January 2019 5,803 4 View
I've performed an ELISA on conditioned media of protein secretion. This assay was done on a serially diluted sample set with dilution factor of 6. However, when accounting for the dilution during...
11 November 2018 4,089 2 View
I live in Durban South Africa.
31 January 2018 4,707 4 View
Hi, I'm researching a treatment and using the CMAI short form as a measure of participant agitation. The CMAI short form breaks questions into 3 categories of agitation. I administered the CMAI at...
21 November 2017 5,284 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View