I have extracted plant samples using four different solvents. I found that the DPPH scavenging activity of all tested samples are averagely similar but the FRAP reducing result of a particular sample seems to be very much higher than the rest of the samples. What does this signify? How can I interpret this result?
Colour controls are OK, but I guess the question is how to discriminate whether or not the observation of high FRAP activity in the sample is real, or there is an artefact. As redox reactions are involved, we should be sure there is not pH change among the assays of the different sanples when FRAP reagents is used.
actually both technique tells about phenolics content present in the sample which directly shows the antioxidant activity.
in DPPH phenolics scavange the DPPH radical which inturns shows amount of phenolics present.
In FRAP fe makes chelate with phenolics with redox process which further tells about phenolics.
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