Animals were fed DSS via drinking water and fecal samples were collected and DNA was isoklated using Qiagen stool DNA isolation kit. The extracted DNA was sent to a sequencing lab to be analysed for microbiota using diversity profiling/sequencing. The DNA sample however failed the QC. Next, I realized that DSS is a PCR inhibitor and hence interferes with the process. Did anyone face similar issue and how did you resolve this. Any advise will be much appreciated.
DNA can easily be precipitated by different methods, such as ethonal, isoproponal, or peg etc. it is unlikely your DSS will also be precipitated by all of these method.
Thanks . Could you please provide a protocol for the same if possible. Thanks.
I use to used the E.Z.N.A. Stool DNA Kit (OMEGA) for extract DNA from fecal of rat that received carcinogen (DSS+AOM).
Maybe it is to late. But I am attaching two SOP that you can use to extract DNA from faeces. Should work because we use this protocol with animals treated with DSS as well.
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