any suggestions relating to the streak seen on the gel (run for plasmid) for the recombinants observed against the band for control plasmid.. and any detail in reference to the cloning process per se..can any one relate to
your question is not clear, write clerly with some detail of protocol adapted,
M15 is expressing strain, what is the problem please elaborate....for propagation and cloning u can use Dh5alpha.
thanx all for your response ..will get back shortly.
Source is QIAGEN and currently the host strains cannot be procured. And if anybody can clarify if the lacIq strains can be good enough? Thanx.
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