Source is QIAGEN and currently the host strains cannot be procured.
And if anybody can clarify if the lacIq strains can be good enough?
Thanx.
Yes, any strain that produces laciq is enough
QIAexpress pQE vectors and constructs can be maintained in any E. coli strain that is ampicillin-sensitive and carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome. (https://www.addgene.org/vector-database/3875/)
XL1-Blue, XL10-Gold, Shuffle, etc.
Thanx much all!!
Is the XL1-Blue strain good enough for expression or should be just used for propagation of pQE30 vector??
At least, XL1-Blue can be used for the expression of mesophilic DNA polymerase in soluble form.
I will suggest you to use M15 strain that is QIAExpress series and recommended for PQE30 series
Thanx all.
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