Monica Sharma

2 Questions 5 Answers 0 Followers

Questions related from Monica Sharma

Can anybody suggest alternate host for a PQE30 vector, a T5 promoter expression vector requiring a trans repression plasmid bearing host E.coli M15?

Source is QIAGEN and currently the host strains cannot be procured. And if anybody can clarify if the lacIq strains can be good enough? Thanx.

08 August 2015 2,438 6 View

Can anyone advise me on cloning and standardizing pQE30 in bacteria other than e coli M15?

any suggestions relating to the streak seen on the gel (run for plasmid) for the recombinants observed against the band for control plasmid.. and any detail in reference to the cloning process per...

02 February 2015 3,328 3 View