Hi everyone we are in the process of standardizing the micro-propagation protocol of culinary banana (ABB) for commercial scale production. But, unlike Grand Naine and other table varieties of banana these culinary types are showing more elongation, lanky and week growth while hardening (Picture attached). Further we also recorded low survival in field condition. In this contest i am planning to use some additional media supplements in in vitro (may be before in vitro rooting step) to improve the plant health condition there by more field survival. I am requesting the experts to suggest the media supplements to produce the good quality planting material.
Hello,
I think those articles are going to be useful for you:
https://pdfs.semanticscholar.org/f373/96f13fee7f381c84c27ee957472c8076eadc.pdf
Article In vitro propagation of banana (Musa spp.): initiation, prol...
https://scialert.net/fulltextmobile/?doi=ajps.2004.406.409
Article In vitro action of ethylmethanesulphonate on banana shoot tips
https://www.researchgate.net/publication/45725805_In_vitro_Shoot_Multiplication_and_Rooting_of_Banana_cv_Sabri
Article In vitro propagation of Banana (Musa sp - Rasthali variety) ...
Have you already tried any of those methods suggested?
Best
Sir, Thank you for quick reply. But the information which i needed is not available in the articles tagged by you. We have developed standardized protocol for ABB group but the resultant shoots were lanky and not vigorous. So i want to use some of the chemicals like Chitosan, Paclorobutrazol, high Sucrose, Silver nitrate etc. to improve the vigor of the micro-shoots by adding these supplements in one or two cycles in in vitro. I am in search of other additives for producing healthy and sturdy tissue culture plants in commercial scale.
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