Can anybody suggest me appropriate tool for aligning and assembly of 574 sequences of 68 Mb data together?

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Does Gaussian 09W software take too long to give a result if we have a large chemical structure ?

DFT - gaussviev - gaussian09W - HOMO - LUMO

08 February 2021 6,012 5 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

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Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

In DFT or HF, is it more expensive to calculate the H matrix or to diagonalize it?

I was wondering: in common DFT (or Hartree-Fock) algorithms/codes, and in particular in solving the Roothan equation, what is the most computationally expensive part: 1- evaluate all the terms of...

01 February 2021 396 2 View

Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

Stephen Tsoi

you can try to use galaxy https://usegalaxy.org/

Ratnesh Tripathi

Thankyou for your suggestion...

May I know if galaxy takes PacBio long read partial assembled data of 68Mb for aligning and assembly?

I am not familiar with PacBio NGS plateform and not sure galaxy has deveopled long read from PacBio. You can send your question to them. If not, Lasergene from DNAStar will do. They have input data for PacBio. Talk to them. GoodLuck!

Thea Kosche

I had a look at the PacBio webpage and they give some suggestions. Here's the link: http://www.pacb.com/products-and-services/analytical-software/ and that's where one can download the software: http://www.pacb.com/support/software-downloads/ and here are some tutorials: http://www.pacb.com/products-and-services/analytical-software/devnet/

I hope this helps.