There are some kit to determine concentration of beta, alpha - carotene. Beside, but also there method to discover total carotene, alpha, beta - carotene. You can follow the paper below.
Total carotenoid content, α-carotene and β-carotene, of landrace pumpkins
(Cucurbita moschata Duch): A preliminary study.
L.M.J. de Carvalho et al. / Food Research International 47 (2012) 337–340
I have been measure the total of beta-carotene,, but i want to measure encapsulation efficiency of beta-carotene in dextrin.
I make the product from beta-carotene, and then i will do encapsulation to my product...And now, i want to measure the encapsulation efficiency from my product..But i found yet the method..
I have encapsulated beta-carotene by maltodextrin and I have determined encapsulation yield and encapsulation efficiency to estimate the processus. For encapsulation efficiency, I have determined it as ratio of encapsulated beta-carotene (total BC in capsule - BC on the surface of capsule) and total BC in capsule. For total BC, I extract it with solvent 3 or more times until colorless. For BC on surface, I put 0.5 g of encapsulated powder in a glass tube then add 5 mL of n-hexane and mix for 5 min, recupere the solvant and mesure BC concentration. I have used this protocol for BC encapsulated in maltodextrin, gelatine, chitosan and carrageenan and it worked well. So I think you could try it. Best regards.