How Triton-x removes SDS from SDS-Polyacrylamide gel?

I came to know about removal of SDS from Polyacrylamide gel is done by Triton-X, but what is the exact mechanism of its removal. How Triton-x does that? Thank you

03 February 2021 7,206 2 View

How can we differentiate crystalline peak to an amorphous peak of XRD ?

When characterized with XRD , my prepared sample show peaks which are broader(bottom) than crystalline peaks but unlike amorphous materials, have sharp top. Is there any limit of intensity or full...

15 December 2020 671 4 View

Can enzyme that is extracted from Aspergillus niger be used in the range of 4-40% v/v for optimization of veggie juices like broccoli??

09 December 2020 7,083 3 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

Regarding FAR, FRR, EER curves?

I have the image database, but I need to calculate the FAR, FRR, and EER and plot them. I am unable to understand how to plot them as they are the images... I am unable to make a program using the...

05 October 2020 3,459 3 View

Can anyone help me out to find SMA connector for a 5g millimeter-wave antenna of dimension 10*10 mm ?

Hello everyone. I am looking for an SMA connector for a 5g millimeter-wave antenna of dimension 10*10mm. The frequency range of operation is up to 40 GHz. If anybody from India is working in this...

29 August 2020 5,275 5 View

How to convert sdf file to pdb or mol2 file ?

I am using Autodock4 for protein-ligand interaction. I need a pdb or mol2 file for ligand. I have downloaded sdf file and convert it in pdb and mol2 file by using save as option. But these files...

23 February 2020 8,819 7 View

Wildtype plant DNA concentration and dilution?

I have 10 rice samples which I'm testing with housekeeping primers. 9 of them are supposed to be transgenic. And 1 is wildtype. I did a PCR with all of them, in which I had a band with all the...

09 February 2020 9,323 3 View

How to quantify biofilm biomass, maximum thickness, and surface to volume ratio from CLSI image, using Image J??

I tried using COMSTAT plugin in Image J, but it is unable to analyse .czi format. Can anyone help?? Or is there any other way to analyse biomass, maximum thickness, and surface to volume ratio...

03 December 2019 3,634 4 View

How do devices live in their own reality based on the information that has been derived?

This question is for my thesis which exploring the internet infrastructure, internet of things and aspects of object-oriented ontology in building device-device relationships.

22 September 2019 2,367 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View