https://www.researchgate.net/publication/8361782_Robust_simple_sequence_repeat_markers_for_spruce_%28Picea_spp.%29_from_expressed_sequence_tags?ev=prf_pub
"Robust simple sequence repeat markers for spruce (Picea spp.) from expressed sequence tags". Dainis Rungis, Yanik Bérubé, Jun Zhang, Steven Ralph, Carol E Ritland, Brian E Ellis, Carl Douglas, Jörg Bohlmann, Kermit Ritland
Article Robust simple sequence repeat markers for spruce (Picea spp....
I suppose your insect's genome is not sequenced? In this case I can suggest some papers now and if that is not enough for you come back with additional question and I will try to answer. The DOIs or links of the papers are: 10.1111/j.1365-2583.2007.00757.x
10.1016/j.tibtech.2007.07.013
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1205583&tool=pmcentrez&rendertype=abstract
http://www.ncbi.nlm.nih.gov/pubmed/10560968
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1460615&tool=pmcentrez&rendertype=abstract
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2948009&tool=pmcentrez&rendertype=abstract
If you have trouble finding the *.pdf write me a message.
Some of the papers will send you to other older papers that might be of help.
Enjoy.
Making two assumptions: 1) you want to develop markers spread evenly across the genome to perform genotyping by PCR and score the genotype by repeat length, and 2) you have an average size insect genome you are dealing with (i.e. in the 200 Mb-1 Gb range) and no draft genome sequence. In the old days we made small insert (1000 base) phage libraries of whole genome, which spread onto plates and did membrane lifts to hybridize to radioactively labelled probes to select clones carrying simple tandem repeats for Sanger sequencing. These days it is much more efficient to use NGS to develop these markers. For example, an average beetle genome of around 700 Mb could be sequenced to sufficient coverage to do some assembly with one run of a MiSeq using 2x300 base reads on a library of average fragment size 450, and using flash or some other read pair joining algorithm you could generate a lot of segments with microsatellites sufficiently centered in the fragment for primer design. Of course, without a full draft genome sequence you would not know where they lie in the genome and would have to construct a genetic map to get relative location.
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