The vectors we have are all integrative ones. a centromeric plasmid may probably make our expression more easy, stable and consistent. Thanks!
Vaskar Mukherjee
Hi Yuke. You can contact Valmik K. Vyas of Finks lab, MIT. My lab has a collaboration with him. So far I know that they are trying to develop a episomal plasmid vector for the delivery of CRISPR Cas in Candida which is already tricky apparently. May be they have a clue about the centromeric system as well. I suppose it is worth to give it a try..
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