Can anybody recommend a suitable supplier etc for water (bottled) to use in an incubator humidity tray (our purified source is not suitable because it is DI, so I'm hoping it will be easier to buy 'ready-made' rather than having to mess around with additives and adjusting pH etc etc)? Thanks!
Hi Glen,
I´m not actually sure if I get the problem. For the incubator I solve 1% copper sulphate in deionised wather and autoclave it. The cooper sulphate is to avoid spore-forming and I use it in incubators made of stainless steel. After autoclaving iI put the water directly in the humidity tray. As I said I hope this what was the question about.
Hi Sibylle,
Many thanks. Yes, I will always add the copper sulphate. My main worry is that I thought DI was not suitable (see bottom of page 4 here: http://www.thermoscientific.com/content/dam/tfs/LPG/LED/LED%20Documents/Application%20%26%20Technical%20Notes/CO2%20Incubators/TNCO2CAREFEED-EN.pdf) but this is all I have 'on tap' and was hoping to avoid having to add NaHCO3, sterilise and test pH etc every time I need it?
Best regards
Glen
Hi Glen,
to be honest, I never read the instructions about the water to use in an incubato. I never had problems with the steel of the incubator because of using DI and I never ajust pH or add NaHCO3 or any other things beside cooper sulphate.
Best regards
Sibylle
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