Hi Georg,

we didn't find a good one as well. If you'll find a suitable one, please post!

Best,

Kai

Hi, We tried several and most don't work. We narrowed on few depending upon the application. Check these papers. We did both Immunoblotting and IHC paraffin.

Effects of selective estrogen receptor agonists on estrogen receptor expression in the uterus of ovariectomized rats. Open Journal of Molecular and Integrative Physiology 05/2012; 2(2).

Expression pattern and signalling pathways in neutrophil like HL-60 cells after treatment with estrogen receptor selective ligands. Molecular and Cellular Endocrinology 2012; 361(1-2):179-90.

Well, thanks Chellakkan Blesson. These antibodies look promising. I'll try them.

It depends whether you are planning to do immunos or Westerns and the species.

For immunos on fixed human tissues the Serotec Mouse anti Human estrogen receptor beta 1, clone PPG5/10 (MCA1974S) is specific for full length protein (antigen retrieval in citrate OK for most tissues). This antibody does not work well for Westerns.

I will chime in that we attempted to validate several widely-used ERbeta antibodies using BERKO mice, but they all show identical patterns of immunohistochemical expression between KO and WT mice. I would strongly recommend that you validate the antibodies with tissues from KO mice before proceeding with experimental tissues.

So just to give you some feedback. Thanks to your responses I bought the PA1-311 from Thermo Scientific.

We checked specificity for ERbeta in IHC using rat liver and rat ovary. In the liver you would not expect significant staining at all and in the ovary only the granulosa cells should be stained. And the results turned out as expected.I am now using it for IHC with rat uterus where I see slight staining, which is the strongest in the epithelium. This is exactly what is to be expected. Furthermore my hypothesis regarding different treatments is supported by the results so I think this AB is working quite well for rat in IHC.

Great to know that the antibody worked! All the best for your experiments.

Is there any update on a good ER-beta antibody especially for Western blotting/ELISA.

Can any be recommended?

RE antibdodies on BERKO it is worth noting that some of these lines of mice retain residual protein expression (spliced variants) so it depends on which line you have and the targeting strategy.