Can anyone explain how to calculate encapsulation efficiency(Plant extract encapsulated alginate nano particles) using total polyphenol content?

Please suggest me if there are any reference materials for this topic. Thank You

30 December 2020 3,799 2 View

Electric field inside a biased N-type uniformly doped semiconductor?

What does the profile of electric field look like in a uniformly doped N-type semiconductor when an external voltage of 'V' volt is applied? Please provide any reference material if possible.

06 November 2020 1,987 8 View

Plane stress or plane strain for Railway Ballast system ?

Dear All, I am working on a wooden rail sleeper analysis. Using the Druker Prager model for Ballast and soils, Initially, I assumed the 2D Behavior of RailSleeper and Rails was Plane Stress and...

04 October 2020 5,459 8 View

What should be Optimum Review duration for a research/review article for any [unpaid/freely publishing]journal till acceptance?

Dear Research Gate Members, We all have experience regarding publications of research/review articles in various journals. Some of the journals review the articles in 4-6 weeks and some takes...

01 October 2020 7,201 1 View

How to write an impressive review paper in the field of Industrial effluent treatments?

I am currently working on collecting literature based on the available treatment technologies for industrial effluents. There are vast variety of methods physical, biological and Advanced...

23 September 2020 2,314 4 View

Fibrous rock with triclinic symmetry?

I am working on wave propagation in fiber reinforced material. Please provide me an example of rock-type which is consisting property of fiber and exhibiting triclinic symmetry with its elastic...

20 September 2020 9,874 1 View

Can someone point me towards a commercially available E1b19K antibody against Human Ad5-E1b-19K?

#antibody #western #Ad5 #E1b19K

16 August 2020 9,283 1 View

How to pursue modelling and map Traditional Knowledge?

I was wondering is it possible to map Traditional Knowledge (TK) of a bigger region (say staus of TK) on the basis of sampling in a smaller region through Arc GIS and modelling? Say we sampled in...

13 August 2020 308 3 View

Pressure behavior from CFD simulation and testing?

Hello, I am simulating gas flow from a high-pressure (700 bar) to a low-pressure container as shown in the attached figure. The red curve shows the pressure curve obtained from the testing, while...

04 July 2020 2,077 2 View

How Artificial Intelligence and Machine Learning are helping to find COVID-19 drug discovery?

How are Artificial intelligence and Machine Learning boosting COVID-19 drug discovery process?

31 May 2020 2,570 6 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View