How to run Wavelet analysis in Python or R or Matlab?

Can anyone guide by providing the procedure to run and interpret Wavelet analysis in Python or R or Matlab? Thanks in advance

09 May 2020 3,778 8 View

Hi, Has anyone isolated myofibroblasts by magnetic cell beads technique?

06 September 2016 9,414 2 View

What is the importance of ligand partial atom charge and MD simulation?

Does the improper partial atom charge of the ligand have any effect on the MD simulation of the ligand protein complex? If so, will it will affect the calculation of potential mean force of the...

29 March 2014 6,492 4 View

Can anyone help with the differentiation of osteosarcoma?

I'm working with human osteosarcoma cell lines. I would like to know is there any morphological cues with which we can say the cell is undergoing differentiation? Apart from staining.

25 February 2014 2,678 2 View

Principle behind cell fixation?

Can anyone tell me, what is the principle behind cell fixation before any staining and also what happens to cell when we are treating it with the fixing agents like methanol, para-formaldehyde etc?

21 July 2013 5,304 8 View

Endogenous control gene for qRT PCR?

Everyone knows that endogenous control is a yard stick for measuring the expression level of our gene of interest in control and treated samples. Do these endogenous controls vary during...

14 June 2013 6,630 3 View

How to rearrange the data in excel by using formula?

I have a number of column data in MS excel sheet. I need to rearrange them in a single column, but not by copy paste. I need the formula to do it. INPUT: A B C 1 One...

06 January 2013 6,420 2 View

Water mediated hydrogen bond

How can I measure the formation of water mediated hydrogen bonds formed between a ligand and a protein complex during simulation. I'm using gromacs 4.5v. Is there any specific way by which we can...

25 December 2012 4,210 1 View

Hydrophobic contacts

How can i measure the hydrophobic interaction between the ligand and protein using gromacs

16 November 2012 5,175 3 View

Free energy calculation using gromacs

How can we calculate the ligand free energy binding for the trajectory obtained from gromacs ligand protein dynamics

06 November 2012 140 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View