It is possible to identify theses structures under sufficient magnification, with a light microscope. However, it will depend on whether you are looking at isolated or whole muscle. In isolated fibres the NMJ of a rat muscle is quite distinct and appears as a dense, convoluted circular network of "tubes" that lie on the surface of the membrane. Some find that it often resembles a "skull", in appearance.
It is less extensive in humans, compared to rats, and may be hard to identify in a whole isolated muscle. However isolating soleus muscle fibres can be quite hard, so perhaps splitting your soleus muscle a few times longitudinally to obtain a thinner preparation might help you see the strutures better. - With a X20 objective you should be able to see the NMJ, depending on the pre-magnification of you microscope.
If you wish to maintain an intact muscle for other purposes I would suggest that you find a dye/ labled anit-body that will specifically bind to the NMJ and use these to localize and identify the NMJ in whole muscle.
Dear Binney Sharma,
I have no answer to your question. But my research would greatly benefit from your advices. I am trying to write a paper on sleep in obese people and aged people.
My mail: [email protected]
www.remsleep.fr
Alain Gonfalone
128 Chemin de l'Ousière Ferme de la Magdelaine
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Thank you sir I am highly thankful to you for this act of kindness.I want to study the neuromuscular junction in these muscles in rats.Synaptic changes in these antigravity muscles.
Can any body help me to find the concentration of acetylcholine in skeletal muscle NMJ in rats.Also what is the concentration of acetylcholine in these muscles ?
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