Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

After staining a gel with coomassie can I stain the same gel with fluorescent dye?

I want to know if it is possible to be done the inverse to view / verify other bands that not look in staining a gel with coomassie.

01 February 2021 8,647 5 View

What a 50% positive predictive value mean?

I want to explain a threshold of 50% PPV to describe how some assays perform when the prevalence of disease is high or low

07 October 2020 8,657 6 View

Author who is not actually an author of some of my publications?

I came out to realize that a person who is enlisted as an author of some publications that I am the REAL AUTHOR, is marked instead of me. How do I change that? Example: Standardized sampling...

21 September 2020 378 7 View

How to increase the short circuit current density in a perovskite solar cell?

I'm optimizing the procedure for making inverted PSCs: ITO / PTAA(8nm) / CsMAFA(320nm) / PCBM(60nm) / BCP(9nm) / Ag(100nm). The cells look very good, I have a very good FF (0.70) and Voc (1.0V),...

16 September 2020 6,286 8 View

Which primer pair is better for amplifying endophytic fungal communities in roots of trees while avoiding host amplification?

Which primer pair is better for amplifying endophytic fungal communities (metabarcoding with Illumina sequencing) in roots of trees while avoiding host amplification? I would greatly appreciate a...

03 September 2020 728 3 View

No title

Hello, I am looking for the original version of COHIP. To measure quality of life related to oral health, in children.

15 June 2020 5,166 1 View

How to create an index file to make hydrogen bond analysis in gromacs?

Hi everyone. I want to analyze the hydrogen bonds between dppc carbonyl oxygen O22 and cholesterol hydroxyl group O3 H3. However, when I execute the gmx hbond command, it doesn't find the donor...

05 May 2020 3,266 3 View

How to calculate rdf between molecules of the same type in gromacs ?

I want to calculate the rdf between O3 atoms of different cholesterol molecules. Additionally, I want to avoid the calculation of rdf between the same molecule. How can I do this in gromacs ?

30 April 2020 5,064 3 View

What is the correct interpretation for this association between changes in two variables?

Hello everybody! We are carrying out a research where we associate baseline to post-intervention changes between motivation and physical activity variables. My question is the following: In an...

24 March 2020 910 3 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

Historical wind data/map?

Hello All, I am looking for the historical wind data/map (mainly the year 2020-2021). I would like to look at the wind value and direction for the specifics locations within the US for the...

18 February 2021 9,543 4 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

What are the recently methods to estimate low-flow and high-flow time series in ungauged basins?

Hi Is there any new method to estimate ( predict ) low-flow and high-flow regimes in daily scales for ungauged hydrometric stations? Thanks

15 February 2021 3,493 3 View