For image analysis in some applications could be an option, http://gwyddion.net/ or imageJ, these two are open source and multiplatform. could you check the features of each one.
I recommend also this software: http://www.adcis.net/en/Image-Processing-And-Analysis-Software-And-Custom-Engineering-Developments.html It's also not for free bu it's worth the prize.
@E. Garcia-Sanchez, i had down loaded the software, but not getting properly the working procedure. can you help me in getting the tutorial for this software..pls
Just google Fiji, this is an enhanced version of ImageJ. I have used this and found more useful than ImageJ. Perhaps there is also separate tutorial for this.