Captured animals are more susceptible to diseases than an animal in wild. I am trying to isolate microbes from a water body inside a zoo. This water may cause more health risks to zoo animals. Please provide the details of microbes if anybody know so that I can go selectively to culture them.
Thanks in advance!
Thank you Mr Klaus. Mycobacterium is rather difficult to isolate, I think so. Well, I'll try Mycobacterium too. Greetings!
I heard about diarrheogenic Escherichia coli, which is one of the water borne bacteria and as you know, it causes diarrhea
Thank you Mr Waqas. How about Pseudomonas, Klebsiella, Staphylococcus and Serratia??? Can you please give me a suggestion?
Greetings!!
Hello Anila, bacteria of the genus Aeromonas, mainly the motile ones, are very common in water with high level of organic matter. They are responsible of wound infections and some sytemic sindromes (septicemia for example). They exist an especific culture media for this kind of bacteria called "agar aeromonas". Regards
Would the group consider Plesimonas shiglliodes? Common zoonotic concern to staff handling fish and to those animal (marine mammals,bears, penguins that are commonly fed herring)
http://en.wikipedia.org/wiki/Plesiomonas_shigelloides- fish borne pathogen
Brucellosis, salmonellosis and E. coli infection in addition to campylobacteriosis and clostridia such as C.botulinum.
please download my research paper entitle A MODEL TO STUDY THE EFFECT OF BOILING ON WATER BORNE BACTERIAL DIARRHEAL AGENTS
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The most common bacteria that transmitted through water are E.coli, pseudomonus,klebsiella,closteridial spores, brucella, salmonella, campylobacter.
Please do not forget leptospira species. If you have any feral rats or mice they can urinate into the water. As long as the water is not too acidic it could become a risk. Be aware leptospira is serious zoonosis (Weils disease). Best to have a look at a standard text book on standard procedures etc.
You may want to look at Aeromonas veronii as it causes freshwater fish sepsis and ulcer syndrome. This can be isolated from freshwater fishes such as Carassius auratus, Cyprinus carpio, Ctenopharyngodon idella, and Silurus asotus. You would expect the isolates to be gram-negative, rod-shaped, and color yellow on Rimler-Shotts (RS).
Additionally, Plesiomonas shigelloides causes chronic inflammation and abscess formation in some marine mammals. This bacteria can be seen in freshwater ecosystems as well as estuaries. This can be isolated from different freshwater fishes such as Oncorhynchus mykiss, Cyprinus carpio, and Oreochromis niloticus. You would expect the isolates to be gram-negative and rod-shaped and could eaily be grown on routine enteric media such as MacConkey (MAC), xylose lysine decarboxylase (XLD), or Hektoen (HE) agars.
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