i read the term RT-qPCR-D method used in an article "we sought to explore the association between ANRIL and response to treatment in samples from patients with CRC treated with 5-FU by using the RT-qPCR-D method." so want to know about this method.
Hi:, it is a PCR-based technique that couples amplification of a target DNA sequence with quantification of the concentration of that DNA species in the reaction. It is called Quantitative PCR (qPCR), also called real-time PCR or quantitative real-time PCR
thank you for your answer. i know about the RT-qPCR but haven't heard about RT_qPCR-D method. so wanted to know about that." ANRIL promotes chemoresistance via disturbing expression of ABCC1 by regulating the expression of Let-7a in colorectal cancer". you can read the tissue sample results of PCR in this article. the used the term RT-qPCR-D method.
Quantitative real-time PCR (qPCR) is a powerful and ubiquitous method for interrogation of gene expression. Accurate quantification is essential for correct interpretation of qPCR data. However, conventional relative and absolute quantification methodologies often give erroneous results or are laborious to perform.
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